Applying this strat egy to a set of 48 human proteins we demonstrate the scalability of our method and define the expression threshold for detection of knockin cells by flow. Zfns were used to express gfp actb in a cell line already expressing. Homozygous lines carrying the p lat52 gfp transgene were used to express cytoplasmic gfp in the vegetative pt cell twell et al 1990.
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